1.揭示了紧密连接蛋白claudin-2在抵抗细菌感染的具体作用机制。肉鸡感染等应激引起肠道炎症、降低紧密连接蛋白表达，导致肠道屏障功能障碍。为了深入研究肠黏膜上皮细胞间紧密连接的调控作用，建立了同时评价肠道通透性大小的体内多探针检测技术，研究发现病细菌感染通过细胞因子IL-22介导肠道紧密连接蛋白claudin-2表达的提高、增加紧密连接通透性、促进Na+离子和水的排泄，最终实现清除病菌。

2.揭示了肠道基质细胞来源的IL-15参与肠道上皮细胞更新、修复的作用机制。利用肠道organoids 3D培养技术和原代细胞共培养技术，发现肠道组织GP38+基质细胞通过分泌IL-15 促进肠道干细胞上皮更新，肠道GP38+间质细胞来源的IL-15缺失或肠道上皮细胞IL-15Rα基因缺失均可减缓肠黏膜损伤修复。揭示了以分泌IL-15为特征的肠道基质细胞在肠道炎症和损伤过程中通过特异调控STAT-HIF-1α信号参与肠道上皮组织损伤修复的作用和机理，为肠道损伤修复和再生提供了新的调控靶点和思路。

3.构建了调控肠道黏膜屏障功能的饲料营养成分体外高通量筛选平台，从肠道紧密连接、抗氧化、干细胞分化及其微环境调控等4层次筛选靶标成分。

肉鸡日粮添加锌通过ZnR/GPR39-PKCζ和PI3K/AKT/mTOR信号通路促进肠细胞分化及细胞间紧密连接蛋白表达，增强肠道屏障功能，进而改善层上皮细胞的完整性。揭示了锌调控肠黏膜上皮紧密连接、减缓肠道炎症的信号传导机制、丰富了锌营养理论。

日粮添加精氨酸有利于缓解肉鸡坏死性肠炎导致的肠道屏障功能损伤；通过促进肠道基质细胞分泌Wnt2b、激活Wnt/β-catenin信号通路，以及GPRC6A受体促进ILC3分泌IL-22，改善肠道干细胞微环境调控肠黏膜屏障功能。

槲皮素具有良好的抗氧化活性，通过上调GCLC和GR，增加细胞内GSH水平，阻止过多的ROS形成；抑制AQP3、增加NOX1表达，缓解肉鸡肠道氧化损伤和肠道炎症。山奈酚通过TLR4/NF-κB途径抑制肠道血管内皮细胞炎性细胞因子分泌、缓解肠道炎症。解析了黄酮醇缓解肠道氧化应激改善肠黏膜屏障功能的作用机制，开拓降低肠道炎症、实现肉鸡饲料无抗的新途径。

染料木黄酮可通过增加肠道乳酸杆菌的丰度来维持肠道稳态、通过Wnt/β-catenin通路阻止沙门氏菌诱导的肠道杯状细胞丢失和促进粘液分泌，修复感染引起的肠细胞分化稳态失衡和抑制细胞自噬。

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