饲用抗生素的禁用为饲料与养殖业带来新的挑战，具有替抗作用的功能性饲料添加剂需求日益迫切。针对这一问题，开展了抗菌肽分子设计与重组表达、植物提取物对畜禽免疫功能的营养调控技术研究，为安全高效饲料添加剂的研发与应用提供理论与技术支持。

(1)抗菌肽分子构效关系研究及重组制备

从抗菌肽的氨基酸组成特点、结构功能基团和抑菌机理出发，利用生物信息学软件，研究了杂合肽设计的方法与理论，通过杂合方式，进一步提高了抗菌肽的抗菌活性，获得了多个活性更为理想的抗菌肽分子，显示了良好的应用前景和开发价值(Applied Microbiology and Biotechnology，2012)。探讨了抗菌肽分子内的特定结构(铰链结构、Trp等)与抗菌肽活性的关系，为创制高效低毒抗菌肽奠定理论基础(Frontiers in Microbiology，2020;Antimicrobial Agents and Chemotherapy;2016)。在国内较早开展了抗菌肽的重组表达工作，利用大肠杆菌、毕赤酵母、芽胞杆菌表达系统建立多个高效表达抗菌肽的重组菌体系(Process Biochemistry，2016;Biotechnology Progress，2015;Protein expression and purification, 2005)，积累了丰富的试验材料与技术平台，包括抗菌肽的串联、融合与分泌表达，融合小肽切割，多肽分离纯化与鉴定等，为抗菌肽的规模化重组制备进而替代饲用抗生素提供了重要的技术与成果积累。

(2)畜禽免疫功能的营养调控技术

利用植物提取物(白藜芦醇、姜黄素)作为饲料添加剂改善动物的免疫功能，确定了二者在鸡、鸭饲粮中的有效添加剂量，发现二者提高动物免疫功能的作用机制与其提高机体的抗氧化功能密切相关，能够参与宿主防御肽的表达调控，从组织、细胞、分子水平探讨了其调控机制(Poultry Science，2020;Biotechnology and Biotechnological Equipment，2020;Scientific Reports，2015)，同时发现二者能够显著改善鸡鸭的肉品质及风味(LWT-Food Science and Technology，2020)，为其作为饲料添加剂的应用及通过营养技术调控畜产品安全与品质提供理论与技术支持。

发明专利：低毒性人造Cathelicidins肽. 授权号：ZL201510727805，冯兴军，程宝晶，毕重朋，陈志辉，高伟. 授权公告日：2018年7月31日

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