围绕华南地区主要养殖品种石斑鱼和对虾开展研究，在配合饲料养殖效果评价、非粮蛋白源及功能型饲料添加剂的开发与应用方面获得以下成果：

1、冰鲜鱼与配合饲料在石斑鱼中的养殖效果比较

配合饲料营养均衡、质量较稳定、综合利用饲料资源，可提高劳动生产率，冰鲜鱼价值低、食用性差、易致病、易污染环境，但因价格低廉常被用作肉食性鱼类饲料来使用。针对这一现状，研究工作从生长性能、饲料利用、肠道组织结构、消化能力、肠道免疫和肠道菌群等多方面比较冰鲜鱼和配合饲料在石斑鱼的应用效果，并分析两种饲养方式的经济效益，提供详细的参考信息。结果发现，与冰鲜鱼相比，使用配合饲料时，石斑鱼肠道组织学指标、消化能力以及免疫力等指标具有显著优势，经济效益更高。研究成果有助于水产配合饲料的推广使用，对于养殖过程中病害防控及环境保护均有重要意义。

2、非粮蛋白源在石斑鱼中的应用效果评估

针对我国水产动物饲用蛋白质资源日益短缺、鱼粉豆粕等优质蛋白质资源严重依赖进口等制约行业可持续发展的“卡脖子”问题，研发非粮蛋白源的应用技术。研究工作从生长性能、饲料利用、肠道组织结构、肠道免疫和肠道菌群等方面来评估脱酚浓缩棉籽蛋白、花生粕等非粮蛋白源替代鱼粉蛋白对石斑鱼的影响，初步阐明了不同非粮蛋白源影响石斑鱼肠道健康与代谢利用的机制，发现石斑鱼养殖中脱酚浓缩棉籽蛋白和花生粕替代鱼粉蛋白的水平最高可达60 %和50 %。研究成果可为从根本上突破制约我国海水鱼饲料行业可持续发展的瓶颈、促进海水养殖业健康发展贡献力量。

3、对虾功能型饲料添加剂的开发与应用

在水产养殖中，使用化学药物和抗生素等传统的疾病防治方法严重危害水产品的质量安全，对人类健康产生潜在的不良影响。使用功能性饲料添加剂以提高机体免疫力是对虾病害防治的有效措施之一。研究工作以调控重要免疫信号通路为切入点，开发多肽类和小分子化合物类等有效的功能性饲料添加剂，并利用组学技术对各种物质的作用机理进行了深入探讨。现已发现多肽类物质S100可改善对虾的生长性能、抗菌免疫和肠道菌群结构，小分子化合物TWS119可介导GSK3β基因对Wnt信号通路进行调控，进而提高对虾的抗病毒免疫。研究成果不仅可以为对虾类动物病害的免疫防治提供理论依据和方法，也有利于安全有效、环境友好的免疫增强剂的开发，促进水产养殖业的健康可持续性发展。

1、A formulated diet improved digestive capacity, immune function and intestinal microbiota structure of juvenile hybrid grouper (Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂) when compared with chilled trash fish. Guanlin Ye, Xiaohui Dong, Qihui Yang, Shuyan Chi, Hongyu Liu, Haitao Zhang, Beiping Tan*, Shuang Zhang*. Aquaculture, 2020, 523, 735230.

2、Low-gossypol cottonseed protein concentrate used as a replacement of fish meal for juvenile hybrid grouper (Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂): Effects on growth performance, immune responses and intestinal microbiota. Guanlin Ye, Xiaohui Dong, Qihui Yang, Shuyan Chi, Hongyu Liu, Haitao Zhang, Beiping Tan*, Shuang Zhang*. Aquaculture, 2020, 524,735309.

3、GSK3β plays a negative role during white spot syndrome virus (WSSV) infection by regulating NF-κB activity in shrimp Litopenaeus vannamei. Shuang Zhang, Lulu Zhu, Hang Yuan, Sheng Yang, Mustafa Abdo Saif Dehwah, Lili Shi. Frontiers in Immunology, 2020, 11, 607543.

4、Dietary replacement of fish meal with peanut meal in juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂): growth performance, immune response and intestinal microbiota. Guanlin Ye, Xiaohui Dong, Qihui Yang, Shuyan Chi, Hongyu Liu, Haitao Zhang, Beiping Tan*, Shuang Zhang*. Aquaculture Reports, 2020, 17, 100327.

5、Dietary supplementation with polypeptides improved growth performance, antibacterial immune and intestinal microbiota structure of Litopenaeus vannamei. Xuzheng Liao, ShikangHua, BoWang, Haipeng Qin, Jichen Zhao, Zihao He, Xieyan Chen, Yongsheng Liu, Peng Qu, Chengbo Sun*, ShuangZhang*. Fish & Shellfish Immunology, 2019, 92: 480-488.

6、Comparative transcriptome analysis of Litopenaeus vannamei reveals that Triosephosphate Isomerase-Like genes play an important role during Decapod iridescent virus 1 (DIV1) infection. Xuzheng Liao#, Chenggui Wang#, Bo Wang, Haipeng Qin, Shikang Hu, Ping Wang, Chengbo Sun*, Shuang Zhang*. Frontiers in Immunology, 2020, 11, 01904.

7、The T cell factor, Pangolin, from Litopenaeus vannamei play a positive role in the immune responses against white spot syndrome virus infection. Lulu Zhu#, Shuang Zhang#, Cuihong Hou, Xueping Liang, Mustafa Abdo Saif Dehwah, Beiping Tan, Lili Shi*. Developmental & Comparative Immunology, 2021, 104041.

8、Molecular cloning, characterization and expression analysis of ARMC6, ARMC7, ARMC8 from Pacific white shrimp, Litopenaeus vannamei. Shougang Song, Beiping Tan, Xiaohui Dong, Qihui Yang, Shuyan Chi, Hongyu Liu, Haitao Zhang, Shuang Zhang*. Gene,2019,682:50-66.

9、Effects of acute hyperglycaemia stress on indices of glucose metabolism and glucose transporter genes expression in cobia (Rachycentron canadum). Guanlin Ye, Wucai Zhang, Xiaohui Dong, Beiping Tan*, Shuang Zhang*. Aquaculture Research, 2020, 51: 584-594.

10、Functional characterization of a protein inhibitor of activated STAT (PIAS) gene in Litopenaeus vannamei. Shuang Zhang, Chaozheng Li, Wei Wang, Chenggui Wang, Chengbo Sun, Siuming Chan, Lili Shi*. Fish & Shellfish Immunology, 2019, 94: 417-426.